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Dynamic hollow fiber protected liquid phase microextraction and quantification using gas chromatography combined with electron capture detection of organochlorine pesticides in green tea leaves and ready-to-drink tea.

Huang SP, Huang SD

Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.

The dynamic hollow fiber protected liquid phase microextraction (DHFP-LPME) technique was evaluated for the extraction of organochlorine pesticides (OCPs) in green tea leaves and ready-to-drink tea prior to gas chromatography combined-electron capture detection (GC-ECD) analysis. A conventional microsyringe with a 1.5 cm length of hollow fiber attached to its needle was connected to a syringe pump to perform the extraction. The microsyringe was used as both the microextraction device and the sample introduction device for GC-ECD analysis. In this work, the organochlorine pesticides were extracted and condensed to a volume of 3 microl of organic extracting solvent (1-octanol) confined within a 1.5 cm length of hollow fiber. The effects of extraction solvent, extraction time, sample agitation, plunger speed, and extraction temperature and salt concentration content on the extraction performance were also investigated. Good enrichments were achieved (34-297-fold) with this method, and good repeatabilities of extraction were obtained, with full name (RSDs) below 12.57%. Detection limits were much below 1 microg l(-1) for ready-to-drink tea and much below 1 microg g(-1) for green tea leaves.

Published 1 November 2006 in J Chromatogr A, 1135(1): 6-11.
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