Green Tea Research Today is a free monthly online journal that collates and summarizes the latest research about Green Tea, including details on benefits, antioxidants, weight loss, diet, side effects. | ||||||||
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Differential regulation of CD11b on gammadelta T cells and monocytes in response to unripe apple polyphenols.Graff JC, Jutila MA Veterinary Molecular Biology, Montana State University, 960 Technology Blvd., Bozeman, MT 59718, USA. Leukocyte adhesion and migration are mediated partially by CD11b/CD18 (membrane-activated complex-1, CR3). Earlier studies have demonstrated a role for green tea polyphenols in down-regulating CD11b on CD8(+) T cells and monocytes. We have shown recently a stimulatory effect of unripe apple polyphenols (APP) on gammadelta T cells. Thus, we compared the effect of APP on bovine gammadelta T cell and monocyte CD11b expression. Purified bovine monocytes and monocyte-depleted PBLs were cultured with APP. CD11b levels decreased on monocytes in response to APP. In contrast, a gammadelta T cell subset responded to APP by up-regulating CD11b. The CD11b regulation was not seen on gammadelta T cells or monocytes treated with APP fractions depleted of tannins. The APP-induced down-regulation of CD11b on monocytes was inhibited by an anti-CD11b mAb, consistent with previous studies showing that polyphenols bind CD11b. As expected, the anti-CD11b mAb had no effect on the APP response in resting gammadelta T cells, as these cells lacked CD11b. Consistent with the changes in surface CD11b expression, APP-treated gammadelta T cells showed increased adherence to plastic, whereas monocyte adhesion was reduced. APP also induced cytokine gene expression in gammadelta T cells. Some polyphenols are thought of as anti-inflammatory agents; however, these data, as well as other ongoing studies, indicate they have a proinflammatory effect on gammadelta T cells. In vivo, plant polyphenols may enhance gammadelta T cell migration and function at sites of inflammation, where they could induce rapid, immune-regulatory and innate-like immune responses. Published 28 August 2007 in J Leukoc Biol, 82(3): 603-7.
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